Anti-melanogenic activity of different extracts from aerial parts of Nepeta glomeruloasin on murine melanoma B16F10 cells

Authors

  • Atefeh Arab Department of Pharmaceutical Biotechnology, School of Pharmacy; Mashhad University of Medical Sciences, Mashhad, Iran
  • Mohammad Sadeghi Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • rezvan yazdian Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Seyed Ahmad emami Department of Pharmacognosy School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Zahra Tayarani-Najaran Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:

Nepeta glomerulosa Boiss. is a medicinal plant has been used in traditional medicine. The aim of this study was to evaluate the anti-melanogenesis inhibitory activity of MeOH, n-hexane, ethyl Acetate (EtAc), dichloromethane (CH2Cl2), n-butanol (BuOH) and H2O extracts isolated from N. glomerulosa in B16 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different extracts of the plant (50-200 µg/ml) was measured by using MTT, the inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and effect on oxidative stress was determined by the colorimetric and fluorometric method. The data showed that at concentrations our results showed the melanogenesis inhibitory and antioxidant effects of N. glomerulosa on B16F10 cells may recommend a novel agent in diminishing skin pigmentation and skin aging in cosmetic industry.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Anti-melanogenic activity of Viola odorata different extracts on B16F10 murine melanoma cells

Objective(s): In previous studies, antioxidant activity of Viola odorata L. has been demonstrated. In this study, we have investigated the anti-melanogenic effect of extract and fractions of the plant in B16F10 cell line. Materials and Methods: Impact of different increasing concentrations of extract and fractions of V. odorata was evaluated on cell viability, cellular tyrosinase, melanin conte...

full text

Evaluation of antioxidant and anti-melanogenic activities of different extracts from aerial parts of Nepeta binaludensis Jamzad in murine melanoma B16F10 cells

Objective(s): Nepeta binaludensis Jamzad (Lamiaceae) has been used in folk medicine of Iran to cure various diseases. The plant is an endemic species to the country that has recently been identified in Razavi Khorasan province. To evaluate the antioxidant and anti-melanogenesis of N. binaludensis, in this study the inhibitory activity of different extracts of N. binaludensis in murine melanoma ...

full text

Evaluation of Antioxidant and Anti-Melanogenic Activity of Different Extracts of Aerial Parts of N. Sintenisii in Murine Melanoma B16F10 Cells

Nepeta (Lamiaceae) is an important genus with beneficial medicinal properties. N. sintenisiiBornm. has been used in folk medicine of Iran to cure various diseases. We investigated theanti-melanogenesis effects of n-hexane, MeOH, CH2Cl2, n-BuOH, EtOAc, and H2O extractsisolated from the plant in B16 melanoma cells. Various assays including cytotoxicity, mushroomtyrosinase inhibition, inhibition o...

full text

Evaluation of Antioxidant and Anti-Melanogenic Activity of Different Extracts of Aerial Parts of N. Sintenisii in Murine Melanoma B16F10 Cells

Nepeta (Lamiaceae) is an important genus with beneficial medicinal properties. N. sintenisiiBornm. has been used in folk medicine of Iran to cure various diseases. We investigated theanti-melanogenesis effects of n-hexane, MeOH, CH2Cl2, n-BuOH, EtOAc, and H2O extractsisolated from the plant in B16 melanoma cells. Various assays including cytotoxicity, mushroomtyrosinase inhibition, inhibition o...

full text

Evaluation of antioxidant and anti-melanogenic activities of different extracts from aerial parts of Nepeta binaludensis Jamzad in murine melanoma B16F10 cells

OBJECTIVES Nepeta binaludensis Jamzad (Lamiaceae) has been used in folk medicine of Iran to cure various diseases. The plant is an endemic species to the country that has recently been identified in Razavi Khorasan province. To evaluate the antioxidant and anti-melanogenesis of N. binaludensis, in this study the inhibitory activity of different extracts of N. binaludensis in murine melanoma B16...

full text

Anti-melanogenic activity of Viola odorata different extracts on B16F10 murine melanoma cells

OBJECTIVES In previous studies, antioxidant activity of Viola odorata L. has been demonstrated. In this study, we have investigated the anti-melanogenic effect of extract and fractions of the plant in B16F10 cell line. MATERIALS AND METHODS Impact of different increasing concentrations of extract and fractions of V. odorata was evaluated on cell viability, cellular tyrosinase, melanin content...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 13  issue 2

pages  61- 74

publication date 2017-04-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023